The phage antibody library technology emerged in the late 1980s, a new antibody preparation, antibody genes can be easily obtained. In autoimmune diseases, cancer and other diseases of the immune antibody library has been applied and achieved satisfactory results. Storage capacity and the diversity of antibodies in the antibody library is screened by the specific antibodies. Capacity the greater the richer the diversity of antibody libraries, from the possibility of antibody screening to the greater. Antibody library build process, most researchers have sought to build the capacity of the antibody library, but less attention on the diversity of the antibody library. The study were collected from 25 patients with 50 mL of peripheral venous blood in patients with mixed peripheral blood lymphocytes were separated and extracted total RNA, and the application of the primer has been proven to be amplified by all antibody variable region fragments, to ensure the diversity of antibody libraries sex. The PCR method selected and the set of conditions is whether the amplification of antibody variable region gene sequences is another important factor. The experiments using semi-nested PCR amplification. Using semi-nested PCR amplification in the second round of the introduction of the corresponding restriction sites in preparation for the next cloning. Primers used by the author more, if all the direct PCR requires a lot of samples, the use of semi-nested PCR can take advantage of the product in the first round as the second round of PCR template, and significant savings in the use of cDNA . Of references in the experiment to introduce four restriction sites, and almost not in the corresponding light and heavy chain variable region appears to avoid the abnormal recombinants in the digestion process, to ensure the accuracy and specificity of the experimental results. In summary, the study was successfully constructed pneumoconiosis phage antibody library, and storage capacity and diversity of the antibody library is guaranteed, and lay the foundation for for pneumoconiosis antibody screening, pneumoconiosis immunotherapy provides a new ideas.
Pneumoconiosis is still of the highest incidence of death more dangerous, the most serious harm to workers occupational disease, so far there is no elimination of or reversal of the cure, only the condition of patients comprehensive medical treatment to alleviate the symptoms.delay the progress of the disease, therefore, active prevention and early diagnosis is particularly important to enhance patients' quality of life and prolonging life. Home and abroad have a lot of research on pneumoconiosis, but the formation mechanism of pneumoconiosis is not entirely clear, then dust population health surveillance, early warning and early detection of pneumoconiosis effective biological indicators for monitoring the lack of the diagnosis still relies on condition to achieve certain pathological extent of the X-ray findings. Further explore the occurrence of pneumoconiosis, development and evolution process, screening and identification of the dust exposure can reflect, the pneumoconiosis process biomarkers, explore the molecular mechanism of the morbidity of pneumoconiosis, early warning and diagnosis and intervention target to prevent fibrosis progression point has a positive significance and role. Scholars proposed a variety of pneumoconiosis research strategy, researchers on the relationship between pneumoconiosis and immune function and has been made a lot of laboratory and epidemiological studies of pneumoconiosis are associated with immune function changes, in view of pneumoconiosis incidence of pneumoconiosis of the lung tissue has a certain antigen composition, dust exposed workers, pneumoconiosis patients in vivo existence of certain autoantibodies, screening can reflect of pneumoconiosis contact or pneumoconiosis flag antibody in the development process, the interpretation of molecular mechanism the early warning and diagnosis of pneumoconiosis may be of great significance.