Expression of Single-chain Variable Fragment

The single-chain antibody (single-chain variable fragment, scFv) by an appropriate period of oligonucleotides (Linker) antibody light chain and heavy chain variable region gene at the DNA level will be linking in vivo performance of a singlepeptide chain, with the minimum structural unit of antibody activity, and connected with a variety of effector molecules to construct the ideal components of the new function of antibody molecules, is one of the hotspots in the field of genetically engineered antibodies. Today, scFv gradual replacement of the monoclonal antibody (mAb) to become a valuable molecules for the treatment and detection of diseases such as cancer, inflammation, autoimmune diseases, hepatitis B and AIDS, biological pathogens, send

Health insect and microbial contamination prevention and detection has great application prospects. Kim et al. Exceed the superparamagnetic iron oxide nanoparticles (SPI-ONs) and scFv composed of abf-SPIONs, the treatment of carcinoembryonic anti-(CEA) has a potential role. Shanta and other applications for the amyloid precursor protein (APP) of scFv to inhibit the activity of β-secretase enzyme of Alzheimer's disease cells to solve the difficulties of the mAb in the treatment process. scFv expression in different expression systems, including the prokaryotic expression system (such as E. coli, Bacillus subtilis) and eukaryotic expression systems (such as Saccharomyces cerevisiae, Pichia pastoris, insect cells, plant cells and animal cells). Affect scFv expression yield and activity of many factors, such as the size of the scFv was, solubility, stability and amino acid sequence. Therefore, according to the requirements of different types of scFv expression and the purity and quantity of the final product required in the expression of scFv to choose the best expression system

16.8.12 18:13


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